Fig. 6.
Effects of protein synthesis inhibitors (act-D and Chx), monensin, and U73122 on DEX-induced apoptosis.
Thymocytes pretreated for 30 minutes with 2.5 μg/mL act-D or 50 μg/mL Chx (A) or with 2.5 μM U73122 or 10 μg/mL monensin (Mon.; B) were cultured for 18 hours in the presence of DEX (10−7 M). Apoptosis was evaluated by PI staining and FACScan flow cytometry. Hydrolysis of SM was evaluated in untreated and DEX-treated thymocytes, not pretreated or pretreated with U73122 or monensin (C). Mean values ± SE of 3 different experiments in duplicate are reported.
