Fig. 3.
DEX activates caspase-3, -8, and -9.
(A) Murine thymocytes were untreated or treated for the indicated times with DEX (10−7 M, 1-6 hours). Caspase activation was assayed by Western blot analysis with the appropriate antibodies. (B) Thymocytes were pretreated for 30 minutes with the indicated caspase inhibitor and were cultured in the presence or absence of DEX (10−7 M, 18 hours). Apoptosis was evaluated by PI staining. Mean values ± SE of 3 different experiments in duplicate are reported.
