Phenotype of EL4 cells.

(A) Flow cytometry analysis of in vitro–grown EL4 cells stained with monoclonal antibody against Thy1.2, CD4, CD8, and TCR Vβ12. Numbers in graphs correspond to the percentage of positive cells. (B) Negative control showing the virtual absence (0.01%) of Thy1.2⁺CD4⁻CD8⁻Vβ12⁺cells in normal B10 splenocytes. (C) Detection of cells with the EL4 phenotype (3.52%) in the spleen of a B10 mouse 15 days after injection of 10⁵ EL4. In panels B and C, cells in R1 were stained with antibodies against Thy1.2 (R2), CD4, and CD8 (R3) and Vβ12 (R4). Cells present in R1 + R2 + R3 + R4 are depicted in the rightmost panels.