C3a induces calcium flux in lineage-expanded hematopoietic cells.

Normal human BM Fura-2–loaded MNCs (A), myeloblasts (B), erythroblasts (C), and megakaryoblasts (D) were stimulated by C3a (1 μg/mL) added at the indicated time points, and calcium flux was evaluated by spectrophotofluorimetry. Data presented are from a representative experiment, which was repeated 5 times for BM cells and 3 times for myeloblasts, erythroblasts, and megakaryoblasts with similar results.