Fig. 7.
Analysis of VH gene use in EU12 subclones.
(A) 4 EU12 subclones derived from sorted single cells with pro-B phenotype were selected on the basis of their phenotypic profiles after 10 weeks in culture. (B) gDNA was isolated and used as template for PCR reactions as described for single-cell PCR analysis. The PCR products were subjected to electrophoresis and revealed with ethidium bromide staining.
