Sequence analysis of Ig HC and LC genes in μHC⁺ EU12 cells.

The cDNA of sorted μHC⁺ EU12 cells was used as template for PCR amplification at V_H-Cμ (A) and V_L-Cλ (C) transcripts using primer combinations described in “Materials and methods.” (B) Single μHC⁺ EU12 cells were sorted into PCR tubes with lysis buffer, and used for 2-round genomic PCR as described in “Materials and methods.” PCR products were purified and directly sequenced with specific V_H primers.