Stat1 phosphorylation is augmented in IFNγ-stimulated

Fancc^−/− macrophages.Peritoneal macrophages from control andFancc^−/− mice were stimulated with IFNγ and their cell lysates subjected to Stat1 immunoblotting analysis. (A) Representative filter showing phospho-Stat1 (top panel) and total Stat1 protein (bottom panel). (B) Densitometry analyses of 4 independent experiments demonstrated a significant increase in the phospho-Stat1 signal within Fancc^−/− macrophages at 15 minutes after stimulation with IFNγ. *P < .05.