Relative expansion and cell cycle analysis of AML1-ETO–transduced erythroid progenitors cultured in IL-3, IL-6, and SCF.

(A) Expansion of CD13⁻ cells during the EPO-independent phase of growth (see “Materials and methods”). Data indicate mean ± 1 SD (n = 4; where n represents the number of independent experiments). Error bars represent 1 SD. (B) Representative histograms (from 1 of 3 experiments) showing cell cycle distribution of AML1-ETO–expressing cells and equivalent controls after 6 days of culture. The percentage of cells in S + G₂/M is indicated ± 1 SD. Significant difference of AML1-ETO from control cultures (GFP alone) is indicated by the dagger (P < .01).