RTX mediates deposition of C3b(i) on Raji cells in NHS.

(A-D) Cells were opsonized in 50% NHS (panels B,D) or in 50% plasma EDTA (panels A,C) ± RTX (10 μg/mL), and after 3 washes probed with a cocktail of FITC mAb 7C12 and PE mAb HB43. Panel D indicates that binding of RTX to cells in NHS leads to deposition of C3b(i). (E,F) Cells were opsonized with Alexa 633 RTX and Alexa 488 mAb 3E7 during incubation in complete medium (panel E) or in 50% NHS (panel F). Panel F reveals a positive correlation between binding of Alexa 633 RTX and Alexa 488 mAb 3E7 to Raji cells in NHS.