Immature DCs express CAR, MHC class I, and αVβ₅, and can be efficiently infected by replication-deficient adenoviruses.

(A) Immature DCs were examined for the expression of CAR, MHC I, αVβ₃, and αVβ₅ by FACS staining. Cells treated with isotype control antibody (filled histogram) were compared with cells treated with αVβ₅, αVβ₃, CAR, or MHC class I (HLA-A, -B, -C) monoclonal antibodies (open histogram). (B) Immature DCs were left uninfected or infected with Adβ-gal at various MOI, and examined after for the presence of β-galactosidase by FACS as described in “Materials and methods.” (C) Immature DCs were infected with AdGFP at an MOI of 100 and then examined for the presence of GFP by fluorescence microscopy (original magnification for both panels, × 20). Representative phase-contrast and fluorescent pictures are shown. (D) Immature DCs were left uninfected or infected with Adβ-gal, AdNIKdn, or AdIKK2dn at an MOI of 100. After 2 days, cells were lysed and extracts examined for the presence of NIKdn or IKK2dn by immunoprecipitation and Western blotting. An antibody directed against the flag epitope attached to NIKdn and IKK2dn but not β-gal was used as previously described.33 39 In all panels, the results of 1 experiment representative of 3 independent experiments performed on samples from unrelated donors are shown.