American Society of Hematology

Fig. 3.

$Fig. 3. HPLC isolation and purification of the 6 major neutral GSLs in ANLL. / (A) The total neutral GSL fraction from an ANLL M1 (sample 3) was separated by HPLC, followed by HPTLC of individual fractions (fraction numbers 7 through 47). Six major neutral GSLs (bands a-f) were identified. (B) HPTLC of the isolated and purified major neutral GSLs (bands a-f) in ANLL. The total neutral GSL fractions from an ANLL (sample 3) and CML (sample 13) were included as controls. Lane S, commercial GSL size standards ranging from monoglycosylceramides (GSL1, GlcCer), diglycosylceramides (GSL2, LacCer), triglycosylceramides (GSL3, Gb3), tetraglycosylceramides (GSL4, Gb4), and pentaglycosylceramides (GSL5, Forssman). HPTLC solvent, C/M/W 65:25:4 (vol/vol). Stain, DPA reagent.$

HPLC isolation and purification of the 6 major neutral GSLs in ANLL.

(A) The total neutral GSL fraction from an ANLL M1 (sample 3) was separated by HPLC, followed by HPTLC of individual fractions (fraction numbers 7 through 47). Six major neutral GSLs (bands a-f) were identified. (B) HPTLC of the isolated and purified major neutral GSLs (bands a-f) in ANLL. The total neutral GSL fractions from an ANLL (sample 3) and CML (sample 13) were included as controls. Lane S, commercial GSL size standards ranging from monoglycosylceramides (GSL1, GlcCer), diglycosylceramides (GSL2, LacCer), triglycosylceramides (GSL3, Gb₃), tetraglycosylceramides (GSL4, Gb₄), and pentaglycosylceramides (GSL5, Forssman). HPTLC solvent, C/M/W 65:25:4 (vol/vol). Stain, DPA reagent.

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