Immunologic detection of neolacto- and globo-family GSLs, including Gb3, in acute myeloid leukemia.
Neutral GSLs from ANLL (lanes 1-11), CML (lanes 12 and 13), and peripheral blood neutrophils (N), lymphocytes (L), platelets (P), and red cells (R) were separated by HPTLC and chemically stained with DPA or immunostained with carbohydrate-specific MoAbs and lectins (Table4). (A) DPA spray, (B) MoAb TE5, (C) MoAb IB2, (D) MoAb Pk002, (E) MoAb MC631, (F) MoAb Gal01, and (G) Stx. Lane numbers for ANLL and CML refer to individual sample numbers shown in Tables 2, 3, and 5. Numbers to left of figures refer to the relative mobility (Rf) of specific GSLs. GSL controls included galactosylceramide (GalC, panel F) and kidney neutral GSLs (K, panel G). Bracketed bands (E, lane 12) represent nonspecific binding. Solvent was C/M/W 60:30:5 (vol/vol).
