Expression of embryonic and adult globin in erythroblasts derived from ES cells in vitro.

Immunostaining of εy and β-major globin was described in “Materials and methods.” (A) Incubation of day 8 erythroblasts with rabbit antimouse εy globin. (B) Incubation of day 8 erythroblasts with rabbit antimouse β-major globin. (C) Incubation of day 14 erythroblasts with rabbit antimouse εy globin. (D) Incubation of day 14 erythroblasts with rabbit antimouse β-major globin. Original magnification × 1000.