Hierarchical clustering of plasma cell and IgM⁺ B-cell replicates.

The expression of 12 422 genes and 66 hybridization controls were examined in 3 plasma cell (PC) and IgM⁺ B-cell replicates using Affymetrix oligonucleotide array, MG-U74 AV2. Hierarchical clustering was performed using Gene Spring following per gene and per chip normalizations of the average difference. As the color bar indicates, yellow represents the median expression of each gene, normalized to 1.0, and red and blue represent elevated and reduced expression relative to the median, respectively. For genes with a median raw average difference of less than 10.0, per gene normalizations were made relative to 10.0 instead of the median.