Inhibitory effect of IVIg from various commercial sources on constitutive maturation of DCs.

Dendritic cells were generated from healthy donors by culturing monocytes in the presence of GM-CSF and IL-4. At day 5, DCs were incubated with Gammagard, Endobuline, Intraglobin, and Sandoglobulin (0.15 mM) for an additional 48 hours. Percentage down-regulation of MFI of surface molecules on DC after IVIg treatment is shown. DCs cultured in medium alone represented 100% expression. Data are represented as means and standard deviations calculated from 3 independent experiments from different donors.