Flow cytometric analysis of surface molecules expressed by DCs differentiated in the presence of IVIg.

Monocytes were cultured for 5 days with 1000 IU/mL rhGM-CSF and 500 IU/mL rhIL-4 in the presence of IVIg (0.15 mM; right panel) or the absence of IVIg (Ctl, left panel). Surface expression of cellular receptors was analyzed following incubation with relevant mAb or isotype control. Percentage of cells positive for the indicated markers are depicted in upper right corners, and MFI is indicated in parentheses. Results shown here are representative of 3 independent experiments.