Fig. 4.
![Fig. 4. Effect of IL-5 on HSPC migration. / (A) Enriched populations of HSPCs were isolated from WT and IL-5trg mice. WT cells were incubated in media containing the cytokine cocktail (Flt-3 ligand, SCF, IL-6, IL-3, IL-11, thrombopoietin [Tpo]) with or without (control) IL-5. Cells were allowed to migrate toward SDF-1 across an endothelial monolayer for 4 hours. The number of migrated cells was calculated and expressed as a percentage of total cell number. (B) Migrated HSPCs were plated into methylcellulose cultures in the presence of the cytokine cocktail (IL-3, IL-6, SCF, Tpo). The numbers of colonies (n = 4 cultures per group) were counted, recalculated, and expressed as percentage of total migrated CFC mean ± SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/3/10.1182_blood-2002-03-0735/4/h80333792004.jpeg?Expires=1722388482&Signature=vZyU5rrNjUFVzij-zkb6J8VsXsmeD9484ElFawyJl9714M-kT1OXFxQ0sjxNLIqnHxhoUmZYAi2gZGOOiz6M4LSkvM~GS1SJImiXz7qxlCb5uc7G5SFwSQfHYyPR4k1SUYRifc7GHzGM0FsbyxlbTX-6dyWTGyGidTVJ80CQoHRFQddWiSn2GGx0GqF69kL3c4HbkSyjFyJWh46NJNVJX5VK94besqiOnEhLbW~6RDFScijUZLgL6fC5w4kixlS4uSXvnoEnySerXwC9Wn9IbfroNhMZgO4JY9tceLWeymNHlirwYNba68secPSze4lXYRNwUSbHexG~fMRdzVB94g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of IL-5 on HSPC migration.
(A) Enriched populations of HSPCs were isolated from WT and IL-5trg mice. WT cells were incubated in media containing the cytokine cocktail (Flt-3 ligand, SCF, IL-6, IL-3, IL-11, thrombopoietin [Tpo]) with or without (control) IL-5. Cells were allowed to migrate toward SDF-1 across an endothelial monolayer for 4 hours. The number of migrated cells was calculated and expressed as a percentage of total cell number. (B) Migrated HSPCs were plated into methylcellulose cultures in the presence of the cytokine cocktail (IL-3, IL-6, SCF, Tpo). The numbers of colonies (n = 4 cultures per group) were counted, recalculated, and expressed as percentage of total migrated CFC mean ± SD.
