American Society of Hematology

Fig. 2.

$Fig. 2. Separation of the lipid priming activity by phospholipid class from the plasma of day-5 control platelets and day-5 implicated platelets. / Lipids were extracted and separated by means of a normal-phase HPLC system. The resulting 4-minute fractions were resolubilized in 1.25% albumin and tested for their ability to prime the fMLP-activated respiratory burst in PMNs from healthy donors compared with albumin-treated controls. One peak of activity was found at the retention time of lyso-PCs in both the control and implicated WB-PLT units. The figure is representative of 6 control and 6 implicated WB-PLT units, which underwent identical analysis.$

Separation of the lipid priming activity by phospholipid class from the plasma of day-5 control platelets and day-5 implicated platelets.

Lipids were extracted and separated by means of a normal-phase HPLC system. The resulting 4-minute fractions were resolubilized in 1.25% albumin and tested for their ability to prime the fMLP-activated respiratory burst in PMNs from healthy donors compared with albumin-treated controls. One peak of activity was found at the retention time of lyso-PCs in both the control and implicated WB-PLT units. The figure is representative of 6 control and 6 implicated WB-PLT units, which underwent identical analysis.

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