Fig. 3.
Fig. 3. Long-term survival of HIV-specific CD8+ T cells is augmented by IL-15. / (A) Representative flow cytometry showing Annexin V staining of purified CD8+ T cells and HIV-specific CD8+ T cells after cultivation for 7 days ± IL-15. Histograms depict HIV-specific CD8+ T cells gated on tetramer and CD8 stains, CD8+ T cells gated on CD8 staining. (B) Pooled data showing percentages of dead (Annexin V+) CD8+ T cells and HIV-specific CD8+ T cells after 7 days in culture ± IL-15 (n = 8). (C) Pooled data showing absolute numbers of dead (Annexin V+) and live (Annexin V−) CD8+ and HIV-specific CD8+ T cells after 7 days in culture ± IL-15 (n = 8). Bars depict means ± standard errors. The P values were calculated by using Student t test for paired samples.

Long-term survival of HIV-specific CD8+ T cells is augmented by IL-15.

(A) Representative flow cytometry showing Annexin V staining of purified CD8+ T cells and HIV-specific CD8+ T cells after cultivation for 7 days ± IL-15. Histograms depict HIV-specific CD8+ T cells gated on tetramer and CD8 stains, CD8+ T cells gated on CD8 staining. (B) Pooled data showing percentages of dead (Annexin V+) CD8+ T cells and HIV-specific CD8+ T cells after 7 days in culture ± IL-15 (n = 8). (C) Pooled data showing absolute numbers of dead (Annexin V+) and live (Annexin V−) CD8+ and HIV-specific CD8+ T cells after 7 days in culture ± IL-15 (n = 8). Bars depict means ± standard errors. The P values were calculated by using Student t test for paired samples.

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