PP2 and LY294002 inhibit sE-selectin–mediated blood vessel formation in Matrigel plugs in vivo.

(A) A representative assay showing Masson trichrome staining of blood vessels in paraffin sections of Matrigel plugs treated with PBS; bFGF; sE-selectin; sE-selectin + PP2; sE-selectin + LY294002; and sE-selectin + PD98059. Arrows indicate blood vessels. Original magnification, × 40. (B) sE-selectin induced significantly more blood vessel formation (*P < .05, n = 18) compared with the PBS group. The values represent the concentration of hemoglobin (g/dL)/Matrigel-plug weight (mg) ± SEM, with n indicating the number of plugs. The Src inhibitor (PP2) and PI3K inhibitor (LY294002) significantly inhibited sE-selectin–mediated blood vessel formation. However, the MAPK inhibitor (PD98059) failed to significantly inhibit sE-selectin–mediated blood vessel formation. (C) bFGF-induced blood vessel formation was significantly inhibited by the MAPK inhibitor PD98059.