Fig. 1.
Methylation and expression analysis ofp21CIP1 in acute lymphocytic leukemia.
(A) Map of the p21CIP1 CpG island. Each vertical mark indicates a CpG pair. The arrow indicates the initiation of the transcription start site. Region 1 indicates de location of COBRA primers. Region 2 of MSP1 primers, and region 3 of the MSP 2 primers. Note that region 3 corresponds to the area studied by Roman-Gomez et al. (B) Examples of COBRA (region 1), MSP1 (region 2), and MSP2 (region 3) assays. SssItr indicates the methylated positive control. The arrow indicates the restricted methylated band. M indicates methylation-specific MSP reactions, U, unmethylated reactions. (C) RT-PCR reactions. + indicates reactions with reverse transcriptase; –, without.
