Luciferase activities of the hMIP-1α promoter constructs.

The hMIP-1α promoter luciferase reporter constructs were cotransfected with the pTK Renilla luciferase construct into the various cell lines and relative activities (Firefly/Renilla luciferase activity) were determined. The promoter construct containing −671 bp of the MIP-1α promoter significantly enhanced luciferase activity in ARH-77 MM cells, but not in nonmyeloma HL-60, U937, THP-1, Reh, and 293 cells. Similar results were seen in 2 independent experiments (*P < .05). Error bars indicate SE.