Fig. 5.
Induction of specific lysis of the U266 cell line by CTLs by the use of U266 RNA–transfected DCs.
Monocyte-derived DCs of an HLA-A2,−, B18/51, Cw7,− (panel A) and an HLA-A2/3, B35/8, Cw4,− (panel B) buffy coat, transfected with total RNA of the U266 cell line, were used as antigen-presenting cells (APCs) for the induction of CTLs. Cytolytic activity of the CTLs was determined in a standard 51Cr-release assay on day 5 after the first restimulation. Myeloma cell lines LP-1 (HLA-A3/24, B7/18, Cw7,−) and U266 (HLA-A2/3, B7/40, Cw3/7) and the tumor cell lines MCF-7 (HLA-A2,−, B18/44, Cw5,−), SK-OV-3 (HLA-A3/68, B18/35), and A498 (HLA-A2,−, B8,−) were used as targets.
