CDDO induction of cleavage of caspases in a time-dependent manner.

CLL B cells were cultured in the presence or absence of CDDO (1 μM), and cell lysates were prepared at various times. Levels of cleaved caspase-3, caspase-8, and caspase-9 were determined by a protein micro–dot-blot method, with the use of primary antibodies recognizing the cleaved forms of these proteases. Relative levels of cleaved caspases were first normalized relative to β-actin, and then normalized relative to nontreated CLL B-cell cultured at the same time points (means ± SD; n = 3).