PMA induces a rapid calcium influx in Fluo-3/am–loaded erythrocytes.

Cell suspensions of Fluo-3/am–loaded RBCs at 0.8% HCT were treated with 3 μM PMA, 1 μM A23187, or DMSO (negative control). Calcium influx was measured by both (A) fluorescence spectroscopy and (B) flow cytometry. Tracings representative of 11 different donors are shown.