![Fig. 1. Donor T-cell cytokine and cytolytic functions after BMT. / B6D2F1 animals were injected with control diluent (solid bar) or IL-18 (dotted bar) from day −2 to +2, received 13 Gy TBI, and received transplants of 5 × 106 TCD BM and 2 × 106splenic T cells from B6 Ly5.2 (CD45.1+) donor mice. Splenocytes from the recipients (n = 3/group) were harvested on day 14 after BMT, combined and normalized for donor T cells (CD45.1+ and CD3+); and restimulated in quadruplicate with irradiated naive host (B6D2F1) splenocytes in MLR cultures. Supernatants were collected after 48 hours of culture and proliferation was determined by pulsing with [3H]-thymidine (1 μCi/well; 0.037 MBq) for an additional 20 hours. T-cell proliferation (A), IFN-γ secretion (B), and IL-2 production (C) were all similar (solid bar versus dotted bar,P = NS). Results from 1 of 3 similar experiments are shown. (D) Splenocytes harvested from allogeneic animals on day 14 after BMT were pooled (n = 3/group), and normalized for donor CD8+ cells and used in a 51Cr release assay. CTL activity against allogeneic P815 in control (▴) and IL-18 (●) groups was similar; there was no significant lysis of syngeneic targets by either group (IL-18, ■; control, ▪).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/9/10.1182_blood-2002-04-1252/4/h82123340001.jpeg?Expires=1722723828&Signature=XBhkIJjItorcqFFo0IJm4DIsYZeOCOR7X1wWu3Gbvap5x75S7JiM4aN5rbeBrhBVjyYL-fGB6uHaDlLMRmut3p4z3dwkYPiZwjDcW4wdMOC8PkAHmT7i79K6oYt3W~1UNFVIaSKKR8gf5OF3r1xjYVppzAOKi4kAm5S10glvsczdc7xCtxJc-ANOl60jf~Y81y7nQgM2-0ai9f0YzehnpK~WlIk7t2UuEH2w0O-dpP-ZH4EjqfKTlGQRpfs~NtG4FjtFvF7oZZ4QvfAXheyH2QMwvjwcxyipV8dnbE3bovhmep1ON4~~oX-z8Qr4OT63dDYWoz-GfwKokMlhAad2Iw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Donor T-cell cytokine and cytolytic functions after BMT.
B6D2F1 animals were injected with control diluent (solid bar) or IL-18 (dotted bar) from day −2 to +2, received 13 Gy TBI, and received transplants of 5 × 106 TCD BM and 2 × 106splenic T cells from B6 Ly5.2 (CD45.1+) donor mice. Splenocytes from the recipients (n = 3/group) were harvested on day 14 after BMT, combined and normalized for donor T cells (CD45.1+ and CD3+); and restimulated in quadruplicate with irradiated naive host (B6D2F1) splenocytes in MLR cultures. Supernatants were collected after 48 hours of culture and proliferation was determined by pulsing with [3H]-thymidine (1 μCi/well; 0.037 MBq) for an additional 20 hours. T-cell proliferation (A), IFN-γ secretion (B), and IL-2 production (C) were all similar (solid bar versus dotted bar,P = NS). Results from 1 of 3 similar experiments are shown. (D) Splenocytes harvested from allogeneic animals on day 14 after BMT were pooled (n = 3/group), and normalized for donor CD8+ cells and used in a 51Cr release assay. CTL activity against allogeneic P815 in control (▴) and IL-18 (●) groups was similar; there was no significant lysis of syngeneic targets by either group (IL-18, ■; control, ▪).
