Fig. 4.
Binding of FITC-BSA-(5-HT)6 to fibrinogen beads.
(A) Latex beads (6 μm) coated with Fbg were incubated with shaking at room temperature with 4.4 μg/mL FITC-BSA-(5-HT)6. At various times, aliquots were diluted and immediately analyzed for bound fluorescence by flow cytometry. Mean FL1 fluorescence was plotted. Various concentration of N-acetyl serotonin (Ac-HT) were included in the assay and found to inhibit binding of FITC-BSA-(5-HT)6. (B) Data from multiple experiments similar to that presented in panel A were analyzed at 10 minutes (mean ± 1 SD; n = 4) and are plotted as a function of N-acetyl serotonin concentration.
