Fig. 2.
Fig. 2. CMV DNA copy number determination by QC-PCR. / Lysates of 1.3 × 104 cells from a Toledo-infected GM-P culture (MOI of 3, day 10 after infection) were analyzed in the presence of the indicated copies of pON2347 competitor. The positions of the 387-bp product from CMV genomic DNA and the 217-bp product from the competitor template are indicated. The ratio of viral genomic to competitor template was determined by density integration using a Stratagene Eagle Eye II/ Eagle Sight system.

CMV DNA copy number determination by QC-PCR.

Lysates of 1.3 × 104 cells from a Toledo-infected GM-P culture (MOI of 3, day 10 after infection) were analyzed in the presence of the indicated copies of pON2347 competitor. The positions of the 387-bp product from CMV genomic DNA and the 217-bp product from the competitor template are indicated. The ratio of viral genomic to competitor template was determined by density integration using a Stratagene Eagle Eye II/ Eagle Sight system.

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