Fig. 2.
SAHA induces growth arrest and apoptosis in MM.1S cells.
(A) Quantification of proliferation of MM.1S cells using the BrdU incorporation assay, according to the instructions of the manufacturer. (B-F) PI analysis of MM.1S cells that were treated with SAHA (5 μM) in 10% FCS for 24 (C), 48 (D), 72 (E), or 96 (F) hours or controls untreated (B) for 96 hours. The percentage of cells in the sub-G1 region (horizontal bars) indicates significant SAHA-induced cell death. (G) Survival of MM patient cells (average ± SD), quantified by MTT colorimetric assay, after treatment with (■) or without (▪) SAHA (5 μM) for 48 hours. Error bars represent SD.
