Ectopic expression of HA-YY1 in 32D cells inhibits neutrophilic differentiation.

(A) Western blot analysis with anti-YY1 in 32D-WT1 and with anti-HA antibodies in 32D-HA-YY1 cells after switching the cells from IL-3– to G-CSF–containing medium on t = 0 days. The blot was reprobed with anti-actin antibodies for loading control. (B) Differential cell count (blasts, band form, and segmented nuclei) of 2 representative 32D-WT1 and 2 representative 32D-HA-YY1 clones. (C) Micrographs showing morphology of 32D-WT1 and 32D-HA-YY1 clones on day 5 (original magnification, × 1000).