Fig. 5.
![Fig. 5. Effect of hyperhomocysteinemia on LDL uptake. / Mouse peritoneal macrophages were incubated with 20 μg/mL [125I]Ac-LDL or [125I]native-LDL for 2 hours. Protein-associated [125I]radioactivity was measured and normalized as [125I] counts per milligram of protein. The uptake activity of [125I]Ac-LDL in macrophages from CBS−/+/ApoE−/− mice under HF diet was set as the control. LDL uptake activity is expressed as relative cellular [125I]protein (% of control mean values). (Inset) The image of LDLs on 0.75% agarose gel shows the electrophoretic mobility change by acetylation. Values represent mean ± SD from 3 independent assays (n = 9). *P < .01 versus CBS+/+/ApoE−/−; †P < .01 versus CBS−/+/ApoE−/− in the same group.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/10/10.1182_blood-2002-08-2606/4/h81034332005.jpeg?Expires=1723501072&Signature=CU~-9429nweYxTF4pA6L8o1g8u5bwFislyJ47wBPXJqvIXg3IzXQddrr05GqCTlQdBWS2s9-ga83F6mRyb2mb9Q7vZgJhAK1ezBKLFaaZ5KW3R50L~xJcYLHQGg6LnmeZxX8HsCNkQirDSLC~jP7NdNjnCIYxWS8Kys8~DhDZqYyilTByKqwcCE-VHPfwp7T9hmPhUBIqALarR6tYTZh92n6EMCyJHbck-cxGdu8byjSOXuTjeLqsE6PYhxB7xd~1aggWFg~bF9pgAQxQM6PtwOU905qnqFZw4wL3LnMEJSMN5~f5rxWGh4E0f56Hi5X0ecCWsLVYMRP0BFwbAZrqg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of hyperhomocysteinemia on LDL uptake.
Mouse peritoneal macrophages were incubated with 20 μg/mL [125I]Ac-LDL or [125I]native-LDL for 2 hours. Protein-associated [125I]radioactivity was measured and normalized as [125I] counts per milligram of protein. The uptake activity of [125I]Ac-LDL in macrophages from CBS−/+/ApoE−/− mice under HF diet was set as the control. LDL uptake activity is expressed as relative cellular [125I]protein (% of control mean values). (Inset) The image of LDLs on 0.75% agarose gel shows the electrophoretic mobility change by acetylation. Values represent mean ± SD from 3 independent assays (n = 9). *P < .01 versus CBS+/+/ApoE−/−; †P < .01 versus CBS−/+/ApoE−/− in the same group.
