Quantitation of atherosclerotic lesions in mouse aortas.

Atherosclerotic lesion area in aortic arch was measured in Sudan IV–stained aortas and expressed as percentage of aortic arch (A) or percentage of whole aorta (B). The mean lesion areas per each group were shown (n = 5 to 9). Values represent mean ± SD. *P value versus CBS^+/+/ApoE^−/−; †P value versus CBS^−/+ /ApoE^−/−; ‡P value versus CBS^+/+/ApoE^−/− on HF diet; §P value versus CBS^−/+/ApoE^−/−on HF diet. (C) Regression analysis shows the correlation of lesion area in aortic root versus plasma concentrations of Hcy in mice under HF+HM diet. Each dot represents one mouse. r indicates correlation coefficient.