Fig. 6.
![Fig. 6. Effect of Spi-B on T and pDC development in vivo. / CD34+CD38− fetal liver cells were transduced with control-IRES-ΔNGFR and Spi-B-IRES-GFP. After transduction the cells were mixed 1:1 and in total 1 × 106 cells were injected into the thymus transplanted subcutaneously 8 weeks prior to injection of the transduced cells. Two weeks later the mice were killed, the thymus was removed, and single-cell suspensions were made by gently cutting the thymus and pressing the fragments through a stainless sieve. The cells were stained with antibodies against the NGFR, CD123, CD4, CD8, CD45RA, BDCA2, and HLA-DR. We excluded the cells expressing extremely high levels of CD4 and CD123 (CD4+CD123+ cells [8%]) because they may represent an artifact.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/3/10.1182_blood-2002-02-0438/4/h80333763006.jpeg?Expires=1724232026&Signature=3m199EIAZPSRcLrk20Su2OilzewtQyWN7IPg1CEW4gy5L4JwmskqgQF1Ql-H25BAI0VgRE11nlY5k62lqyS-NaLqX6XHrmRnnSEiwhiSm6q84akPmXWm~GatfMycY1HbTHVHAUBUiEFhxJIY~N4DLUdfvg9FGEfPLkcylVneiDTyJugi7VShuIMQUw0I-x~728fNxO8QQQSGkAY8AXeSDsWTXiYt~2mvh4ZEGl~9SCF2~fyi46YTkUnr-35bsKxm8XtNR4WK0YIjOfM~OLbWKBCNkVBK66VJ25LIwSD~YLuhrgg2meOZrRykkGwB-A4TlOZN1shmD-PrXTbwMxjGVw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of Spi-B on T and pDC development in vivo.
CD34+CD38− fetal liver cells were transduced with control-IRES-ΔNGFR and Spi-B-IRES-GFP. After transduction the cells were mixed 1:1 and in total 1 × 106 cells were injected into the thymus transplanted subcutaneously 8 weeks prior to injection of the transduced cells. Two weeks later the mice were killed, the thymus was removed, and single-cell suspensions were made by gently cutting the thymus and pressing the fragments through a stainless sieve. The cells were stained with antibodies against the NGFR, CD123, CD4, CD8, CD45RA, BDCA2, and HLA-DR. We excluded the cells expressing extremely high levels of CD4 and CD123 (CD4+CD123+ cells [8%]) because they may represent an artifact.
