Fig. 3.
Fig. 3. Effect of Spi-B on development of pDCs. / CD34+CD38− fetal liver (A) or CD34+CD1a− postnatal thymus (B) were transduced with LZRS Spi-B-IRES-GFP, LZRS ΔSpi-B-IRES-GFP, or with control-IRES-GFP and incubated with S17 cells for 5 to 7 days for postnatal thymocytes and fetal liver cells, respectively. The quadrants were placed to include 99% of the cells stained with control antibodies. The cell recoveries of all samples were the same; thus, the differences in percentages of pDCs indicated in the figure reflect the differences in absolute numbers of pDCs generated in this system.

Effect of Spi-B on development of pDCs.

CD34+CD38− fetal liver (A) or CD34+CD1a− postnatal thymus (B) were transduced with LZRS Spi-B-IRES-GFP, LZRS ΔSpi-B-IRES-GFP, or with control-IRES-GFP and incubated with S17 cells for 5 to 7 days for postnatal thymocytes and fetal liver cells, respectively. The quadrants were placed to include 99% of the cells stained with control antibodies. The cell recoveries of all samples were the same; thus, the differences in percentages of pDCs indicated in the figure reflect the differences in absolute numbers of pDCs generated in this system.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal