Effect of Spi-B on development of pDCs.

CD34⁺CD38⁻ fetal liver (A) or CD34⁺CD1a⁻ postnatal thymus (B) were transduced with LZRS Spi-B-IRES-GFP, LZRS ΔSpi-B-IRES-GFP, or with control-IRES-GFP and incubated with S17 cells for 5 to 7 days for postnatal thymocytes and fetal liver cells, respectively. The quadrants were placed to include 99% of the cells stained with control antibodies. The cell recoveries of all samples were the same; thus, the differences in percentages of pDCs indicated in the figure reflect the differences in absolute numbers of pDCs generated in this system.