Expression of Spi-B in hematopoietic cells.

(A) A 35-cycle RT-PCR for Spi-B expression was performed on DC2 (lines 1-5), freshly isolated (line 1), after IL-3 activation (for 12 hours, line 2; for 48 hours, line 4), and after IL-3 and CD40L activation, (for 12 hours, line 3; 48 hours, line 5). Monocytes (line 6), Mo-DCs (line 7), and CD40-activated DC1 (overnight, line 8; 48 hours, line 9).