Fig. 6.
Effect of oxygen tension on neutrophil release of IL-8.
Neutrophils (5 × 106/mL) were cultured in normoxia (N; 21%), hypoxia (H; 3%), or anoxia (A; 0%) for 6 or 20 hours as indicated, and supernatants were collected and stored at −20°C. IL-8 release was subsequently analyzed by ELISA. Phytohemagglutinin-stimulated (10 μg/mL) peripheral blood mononuclear cell (PBMC) supernatants were used as positive controls. Neutrophil data represent mean of 9 separate experiments, each performed in duplicate. PBMC control data represent mean ± SEM of 3 separate experiments.
