Fig. 3.
Elevated level of cIAP2 protein in neutrophils from donors receiving G-CSF administration.
Peripheral blood neutrophils were isolated from 4 healthy donors receiving subcutaneous administration of G-CSF (200 μg/m2) twice daily for allogeneic peripheral blood stem cell transplantation. At the indicated days after the start of G-CSF administration, venous blood was obtained just before the injection of G-CSF. The expression of cIAP2, Mcl-1, and p38 proteins was analyzed by immunoblotting using antibodies against each protein. The cell lysates equivalent to 3.7 × 106 cells were loaded onto each lane. Lym indicates lymphocytes (3.7 × 106 cells).
