Fig. 1.
Effects of G-CSF and GM-CSF on expression of apoptosis-related gene transcripts in neutrophils.
Neutrophils were cultivated in the presence or absence of G-CSF (50 ng/mL) or GM-CSF (5 ng/mL) for 1 hour, and thereafter the expression of cIAP1, cIAP2, XIAP, Mcl-1, A1, Bcl-XL, and Bcl-2 mRNA was analyzed by semiquantitative RT-PCR. As internal controls, β2-microglobulin (β2MG) and 18S rRNA were used. The results shown are representative of 3 independent experiments.