Fig. 7.
Fig. 7. IFN-γ production in T lymphocytes induced by KLH-loaded blood CD11c+ DCs, Mo-DCs, and ActMo-DCs. / (A) Autologous CD45RA+CD4+ lymphocyte proliferation and (B) intracellular IFN-γ production induced by 5 × 103 blood CD11c+ DCs, Mo-DCs, and ActMo-DCs (produced in RPMI/5% AS, GM-CSF 200 U/mL, IL-4 50 U/mL and activated with LPS) loaded with 100 μg/mL KLH for 3 hours (DC/T ratio; 1:20). The percentages of T lymphocytes producing IFN-γ are indicated on the dot plots. One representative experiment from the 3 experiments performed is shown.

IFN-γ production in T lymphocytes induced by KLH-loaded blood CD11c+ DCs, Mo-DCs, and ActMo-DCs.

(A) Autologous CD45RA+CD4+ lymphocyte proliferation and (B) intracellular IFN-γ production induced by 5 × 103 blood CD11c+ DCs, Mo-DCs, and ActMo-DCs (produced in RPMI/5% AS, GM-CSF 200 U/mL, IL-4 50 U/mL and activated with LPS) loaded with 100 μg/mL KLH for 3 hours (DC/T ratio; 1:20). The percentages of T lymphocytes producing IFN-γ are indicated on the dot plots. One representative experiment from the 3 experiments performed is shown.

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