Comparison of phenotypic profiles of blood CD11c⁺ DCs, Mo-DCs, and ActMo-DCs.

(A) Blood CD11c⁺ DCs were obtained by lineage negative (Lin⁻) selection using magnetic beads and further sorting for CD11c⁺ cells (R1, a gate used for sorting). Histograms show expression of the cell-surface antigens on blood CD11c⁺ DCs in region R1 (dotted line, isotype control moAb; solid line, specific moAb). (B) Mo-DCs generated as described in “Patients, materials, and methods” had low levels of CD1a expression in most cases. Histograms show expression of the antigens on Mo-DCs and ActMo-DCs (generated in RPMI/5% AS, 200 U/mL GM-CSF, and 50 U/mL IL-4 and activated by LPS) in region R2 (dotted line, isotype control moAb; thin solid line, specific moAb on Mo-DCs; thick solid line, specific moAb on ActMo-DCs).