Expression of hematopoietic markers and that of GFP were reciprocally exclusive.

Sorted Flk-1⁺ cells derived from ES cells were cultured on an OP9 stromal cell layer and analyzed for the expression of hematopoietic markers (CD45 and Ter119; bottom row), an endothelial marker (VE cadherin; top row), and GFP for 5 days. The results shown are representative of 3 independent experiments.