Fig. 3.
Fig. 3. The expression pattern of mSIGNR1 is similar to that of hL-SIGN. / (A) mSIGNR1 is not expressed by bone marrow–derived DCs or by splenic CD11c+ DCs. Bone marrow–derived DCs and splenic CD11c+ DCs were stained with the anti-mSIGNR1 antibody 698. Bone marrow DCs were more than 90% pure as determined by CD11c staining (data not shown). Dotted and open histograms represent isotype and mSIGNR1 staining, respectively. Splenic DCs were double-stained with CD11c and the mSIGNR1-specific antibody 698. (B,C) Immunofluorescence staining of murine liver tissue sections with the polyclonal antibody against mSIGNR1 698 (green, B and C) and double staining with F4/80 (red, C). Original magnification × 200. (D-G) Immunofluorescence double staining of murine lymph node tissue sections with the polyclonal antibody against mSIGNR1 698 (green) and in red CD11c (D), DEC-205 (E), SER-4 (F), MOMA-1 (G). Original magnification × 200.

The expression pattern of mSIGNR1 is similar to that of hL-SIGN.

(A) mSIGNR1 is not expressed by bone marrow–derived DCs or by splenic CD11c+ DCs. Bone marrow–derived DCs and splenic CD11c+ DCs were stained with the anti-mSIGNR1 antibody 698. Bone marrow DCs were more than 90% pure as determined by CD11c staining (data not shown). Dotted and open histograms represent isotype and mSIGNR1 staining, respectively. Splenic DCs were double-stained with CD11c and the mSIGNR1-specific antibody 698. (B,C) Immunofluorescence staining of murine liver tissue sections with the polyclonal antibody against mSIGNR1 698 (green, B and C) and double staining with F4/80 (red, C). Original magnification × 200. (D-G) Immunofluorescence double staining of murine lymph node tissue sections with the polyclonal antibody against mSIGNR1 698 (green) and in red CD11c (D), DEC-205 (E), SER-4 (F), MOMA-1 (G). Original magnification × 200.

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