Structure of the murine chromosomal CD45 gene and the minigene, pLFAiT200.

(A) The map of the 115-kb murine CD45 gene30 shows the exon and intron organization. The 12-kb genomic sequence contained in the minigene is indicated. (B) The structure of the CD45 minigene, pLFAiT200, is illustrated. LFA is the human LFA-1 (CD11a) promoter, and the UTR is the 3′ CD45 untranslated region. The CD45 5′ cDNA region containing exons 1b through 3 and the 3′ cDNA sequence region with exons 9 through 33 are designated 1b-3 and 9-33, respectively. The hatched box indicates the genomic sequence contiguous with the UTR 3′ of the CD45 poly(A) site.