Lentiviral correction of KO mouse hematopoietic tissue.

(A) Colony-forming assays on Fanca^−/−andFancc^−/−mouse bone marrow cells corrected by lenti-FANCA and lenti-FANCC, respectively. Values are mean ± SD of triplicate determinations. (B) Western blot detection of the FANCA and FANCC proteins in the bone marrow of knockout mice that underwent transplantation with lentiviral-corrected cells. For FANCA and FANCC, lanes 1 and 2 are bone marrow samples from primary transplants, lanes 3 to 5 are samples from secondary recipients, and “control” indicates a sample from a knockout control mouse.