Binding of OPG to myeloma cells.

(A) Binding of OPG to CAG cells, (B) primary myeloma cells, and (C) ARH-77 cells transfected with either intact murine syndecan-1 (Syn-1) or with syndecan-1 lacking heparan sulfate side chains (TDM) examined by flow cytometry. OPG/heparin: preincubation of OPG in the presence of heparin; heparin wash: cells incubated with OPG and subsequently washed with heparin; control: cells incubated with PBS instead of OPG.