Activation of caspase-9 and cytochrome

c release in Bcl-2-ASO–treated IM9 cells. IM9 cells were cultured for 72 hours with 0 to 10 μg/mL Bcl-2-ASO. Cultures were harvested and assayed by flow cytometry for caspase-9 activation and cytochrome c release from mitochondria as described in “Materials and methods.” Apoptosis was determined by the annexin V method. At least 3 experiments were performed, and 1 representative experiment is shown. For additional experimental details, see “Materials and methods.” Note similar kinetics for apoptosis, cytochrome c release, and activation of caspase-9. Percentages indicate positive cells.