Bcl-2-ASO down-regulates the expression of Bcl-2 protein in low Bcl-2–expressing cells.

(A) Bcl-2 levels in Bcl-2-ASO–treated IM9 and HS-Sultan cells (low levels of Bcl-2; Figure 1) and U266 and ARH-77 cells (high Bcl-2; Figure 1). (B) Bcl-2 levels in Bcl-2-ASO–treated ARP-1 cells (low Bcl-2, mock-transfected); ARP/Bcl-2 cells (× 10-fold higher Bcl-2; Figure 1); and RPMI 8226 cells and 8226/Bcl-2 cells (× 6-fold higher Bcl-2; Figure 1). Bcl-2–transfected cells were generated and maintained as described previously.9 Cells were treated for 4 days with the indicated concentrations of Bcl-2-ASO (μg/mL). Bcl-2-ASO was replenished every other day. β-actin was used as an internal loading control. Other experimental details are as in Figure 1. Representative results from at least 3 different experiments are shown.