RNA analysis in mononuclear and CD34⁺ cells from RPS19-deficient patients.

(A) RNA was isolated from mononuclear cells of a healthy individual and 4 patients (Patients 1-4, Table 1) with RPS19 mutations and examined by northern blot analysis using a full-length RPS19 probe. No abnormal transcripts were detected. (B, C) RNA from mononuclear cells (B) and CD34⁺ cells (C) was analyzed by quantitative RT-PCR using a LightCycler to amplify RPS19 and actin transcripts. The figures show the signal intensity ratio between the RPS19 and actin transcripts. The Q-RT-PCR experiments in B and C represent the average of 2 experiments.