Normal fMLP-induced degranulation in

syk^−/− neutrophils.Wild-type or syk^−/− neutrophils were preincubated in the presence or absence of 10 μM CB as indicated, and the release of primary or secondary granules in response to fMLP stimulation was determined. (A) Kinetic analysis of the release of the primary granule marker elastase. Cells were stimulated with 3 μM fMLP at 1 minute and lysed with 0.02% CTAB at 4 minutes. (B) Release of the secondary granule marker lactoferrin (Lfr) during a 10-minute stimulation with 3 μM fMLP.