Peptide-binding assays.

Up-regulation of HLA-A0201 expression on T2 cells after preincubation with CD45 peptides (P292-P1218) and a positive control peptide (HBV 18-27) was determined by FACS analysis. Mean fluorescence intensity at varying concentrations of peptide in a representative experiment is shown. In total, 16 CD45-derived peptides were tested. Four peptides showed HLA-A0201 binding (this figure), and 12 peptides showed no binding (this figure and additional data not shown).